Perfil Plasmático de Glicoproteínas asociadas a la preñez durante el período posparto en ovejas Merino Konya con partos simples o dobles

• Eyyup Hakan Ucar Aydin Adnan Menderes University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology. Aydin, Turkey
• Mehmet Kose Dicle University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology. Diyarbakir, Turkey
• Mesut Kirbas Bahri Dağdaş International Agricultural Research Institute. Konya, Turkey
• Faruk Bozkaya Harran University, Faculty of Veterinary Medicine, Department of Animal Science and Animal Nutrition/Department of Veterinary Genetics. Sanliurfa, Turkey
• Mehmet Osman Atli Harran University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination. Sanliurfa, Turkey

DOI: https://doi.org/10.52973/rcfcv-e33298

Palabras clave: Konya Merino, glicoproteínas asociadas a la preñez, período postparto

Resumen

En este estudio, se investigó el Perfil Plasmático de las Glicoproteínas asociadas a la preñez (PAGs) después del parto en ovejas Konya Merino utilizando un kit de prueba de preñez utilizado en el ganado vacuno. Se utilizó un total de 16 ovejas Konya Merino como material. Se establecieron cuatro grupos de ovejas que parieron a un cordero macho (grupo SM, n=4), una cordera hembra (grupo SF, n=4), corderos mellizos machos (grupo TM, n=4) o corderas mellizas hembras (grupo TF, n=4). Se tomaron muestras de plasma sanguíneo en los días 0 (día del parto), 3; 7; 10; 14 y luego semanalmente hasta el día 35. Todas las muestras se analizaron utilizando un kit de prueba de preñez bovino para detectar el nivel de PAGs. Se determinó que existe una fuerte correlación negativa entre el nivel de PAGs en la sangre periférica y los días después del parto (r²=0,969; P<0,01). Sin embargo, no se encontró ninguna relación entre el nivel de PAGs y el género del cordero o el tipo de parto. En conclusión, el nivel plasmático de PAGs disminuyó rápidamente en ovejas Konya Merino independientemente del género y tipo de parto del cordero. Además, los resultados mostraron que el perfil plasmático de PAGs después del parto en ovejas Konya Merino se puede monitorear con un kit comercial de prueba de preñez bovino basado en ELISA, y los resultados de la prueba se pueden utilizar en decisiones y evaluaciones basadas en los niveles de las moléculas de PAGs.

Citas

Carter AM, Mess AM. The evolution of fetal membranes and placentation in carnivores and ungulates (Ferungulata). Anim. Reprod. [Internet]. 2017; 14(1):124–35. doi: https://doi.org/f9v4pm

Sammin D, Markey B, Bassett H, Buxton D. The ovine placenta and placentitis–A review. Vet. Microbiol. [Internet]. 2009; 135:90–7. doi: https://doi.org/bg447v PMID: 18980813.

Igwebuike UM. Trophoblast cells of ruminant placentas–A minireview. Anim. Reprod. Sci. [Internet]. 2006; 93:185–98. doi: https://doi.org/bdbtwt PMID: 16043315.

Wallace RM, Pohler KG, Smith MF, Green JA. Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy. Reprod. [Internet]. 2015; 149(3): R115–26. https://doi.org/f642st PMID: 25661256.

Szafranska B, Panasiewicz G, Majewska M. Biodiversity of multiple Pregnancy–Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review. Reprod. Nutr. Dev. [Internet]. 2006; 46(5):481–502. doi: https://doi.org/fkj4mn PMID: 17107639.

Ledezma–Torres RA, Beckers JF, Holtz W. Assessment of plasma profile of pregnancy–associated glycoprotein (PAG) in sheep with a heterologous (anti–caPAG55+59) RIA and its potential for diagnosing pregnancy. Theriogenol. [Internet]. 2006; 66(4):906–12. doi: https://doi.org/bt7kdj PMID: 16566995.

de Sousa NM, Zongo M, Pitala W, Boly H, Sawadogo L, Sanon M, de Figueiredo JR, Gonçalves PB, El Amiri B, Perènyi Z, Beckers JF. Pregnancy–associated glycoprotein concentrations during pregnancy and the postpartum period in Azawak Zebu cattle. Theriogenol. [Internet]. 2003; 59:1131–42. doi: https://doi.org/b4c892 PMID: 12527062.

Garbayo JM, Serrano B, Lopez–Gatius F. Identification of novel pregnancy–associated glycoproteins (PAG) expressed by the peri–implantation conceptus of domestic ruminants. Anim. Reprod. Sci. [Internet]. 2008; 103(1–2):120–34. doi: https://doi.org/brzcnw PMID: 17204380.

Roberts JN, May KJ, Veiga–Lopez A. Time–dependent changes in pregnancy–associated glycoproteins and progesterone in commercial crossbred sheep. Theriogenol. [Internet]. 2017; 89:271–279. doi: https://doi.org/kr9k PMID: 28043363.

Ranilla MJ, Sulon J, Mantecón AR, Beckers JF, Carro MD. Plasma pregnancy–associated glycoprotein and progesterone concentrations in pregnant Assaf ewes carrying single and twin lambs. Small Rumin. Res. [Internet]. 1997; 24(2):125–131. doi: https://doi.org/fqfxvc

Kaplan Y, Özyurtlu N, Köse M, Atlı MO, Küçükaslan İ, Kırbaş M. Gebe Konya Merinosu Koyunlarında Erken Gebelikte Gebelik İlişkili Glikoproteinlerin Plazma Profilinin Belirlenmesi. Ataturk Univ. Vet. Bilim. [Internet]. 2019; 14(3):307–314. doi: https://doi.org/kr9m

Haugejorden G, Waage S, Dahl E, Karlberg K, Beckers JF, Ropstad E. Pregnancy associated glycoproteins (PAG) in postpartum cows, ewes, goats and their offspring. Theriogenol. [Internet]. 2006; 66(8):1976–84. doi: https://doi.org/c8x32b PMID: 16870244.

Hooshmandabbasi R, Zerbe H, Bauersachs S, de Sousa NM, Boos A, Klisch K. Pregnancy–associated glycoproteins in cows with retained fetal membranes. Theriogenol. [Internet]. 2018; 105:158–63. doi: https://doi.org/kr9n PMID: 28982025.

Dosogne H, Burvenich C, Freeman AE, Kehrli ME Jr, Detilleux JC, Sulon J, Beckers JF, Hoeben D. Pregnancy–associated glycoprotein and decreased polymorphonuclear leukocyte function in early post–partum dairy cows. Vet. Immunol. Immunopathol. [Internet]. 1999; 67(1):47–54. doi: https://doi.org/db9546 PMID: 9950353.

de Miranda E Silva–Chaves C, Dias da Costa RL, RoncatoDuarte KM, Machado DC, Paro de P. CC, Beltrame RT. Visual ELISA for detection of pregnancy–associated glycoproteins (PAGs) in ewe serum. Theriogenol. [Internet]. 2017; 97:78–82. doi: https://doi.org/kr9p PMID: 28583612.

Rovani MT, Cezar AS, Rigo ML, Gasperin BG, NóbregaJúnior JE, Torres FD, Gonçalves PBD, Ferreire R. Evaluation of a bovine pregnancy–associated glycoprotein enzyme–linked immunosorbent assay kit for serological diagnosis of pregnancy in sheep. Ciên. Rural. [Internet]. 2015; 46(2):362–367. doi: https://doi.org/kr9q

Uçar U, Köse M, Atli OM. Konya Merinosu Koyunlarda Gebelik İlişkili Glikoproteinlerin Gebelikteki Plazma Profili ve Erken Gebelik Tanısında Kullanılabilirliği. Dicle Üniv. Vet. Fak. Derg. [Internet]. 2018 [cited 20 may 2023]; 11(2):77–82. Available in: https://bit.ly/3r34soh.

Echternkamp SE, Vonnahme KA, Green JA, Ford SP. Increased vascular endothelial growth factor and pregnancy–associated glycoproteins, but not insulin–like growth factor–I, in maternal blood of cows gestating twin fetuses. J. Anim. Sci. [Internet]. 2006; 84(8):2057–64. doi: https://doi.org/dtsv25 PMID: 16864865.

Singh SP, Natesan R, Sharma N, Goel AK, Singh MK, Kharche SD. Pregnancy–associated glycoprotein profile in milk and its relationship with the circulating level during early pregnancy in goats. Small Rumin. Res. [Internet]. 2019; 173:81–87. doi: https://doi.org/kr9r

Zoli AP, Guilbault LA, Delahaut P, Ortiz WB, Beckers J–F. Radioimmunoassay of a Bovine Pregnancy–Associated Glycoprotein in Serum: Its Application for Pregnancy Diagnosis. Biol. Reprod. [Internet]. 1992; 46(1):83–92. doi: https://doi.org/cv527c PMID: 1547318.

Sousa NM, Garbayo JM, Figueiredo JR, Sulon J, Gonçalves PBD, Beckers JF. Pregnancy–associated glycoprotein and progesterone profiles during pregnancy and postpartum in native goats from the north–east of Brazil. Small Rumin. Res. [Internet]. 1999; 32(2):137–147. https://doi.org/b89gjf

Barbato O, Menchetti L, Sousa NM, Malfatti A, Brecchia G, Canali C, Beckers JF, Barile VL. Pregnancy–associated glycoproteins (PAGs) concentrations in water buffaloes (Bubalus bubalis) during gestation and the postpartum period. Theriogenol. [Internet]. 2017; 97:73–77. doi: https://doi.org/gbj573 PMID: 28583611.

Steckeler P, Weber F, Zerbe H, Rieger A, Voigt K. Evaluation of a bovine visual pregnancy test for the detection of pregnancy–associated glycoproteins in sheep. Reprod. Domest. Anim. [Internet]. 2019; 54(2):280–288. doi: https://doi.org/kr9s PMID: 30267612.