Detección de Yersinia ruckeri en truchas arcoíris (Oncorhynchus mykiss Walbaum, 1792) infectadas naturalmente en criaderos de truchas en Anatolia, Turquía

• Filiz Özcan Dicle University, Veterinary Faculty, Fisheries and Fisheries Diseases Department. Diyarbakır, Turkey
• Neval Berrin Arserim Dicle University, Veterinary Faculty, Department of Microbiology. Diyarbakır, Turkey
• Muzaffer Aydın Ketani Dicle University, Veterinary Faculty, Department of Histology and Embryology. Diyarbakır, Turkey
• Kasım Aydin Metropolitan Municipality of Diyarbakır. Diyarbakır, Turkey
• Nurdan Karacan–Sever Dicle University, Veterinary Faculty, Department of Microbiology. Diyarbakır, Turkey
• Nida Özcan Dicle University, Medical Faculty, Department of Medical Microbiology. Diyarbakır, Turkey
• Burçak Aslan–Çelik Siirt University, Veterinary Faculty, Department of Parasitology. Siirt, Turkey

DOI: https://doi.org/10.52973/rcfcv-e33280

Palabras clave: MALDI–TOF, Oncorhynchus mykiss, PCR, Yersinia ruckeri

Resumen

El presente estudio tuvo como objetivo detectar la bacteria Yersinia ruckeri en muestras de pescado recolectadas de las granjas de trucha arco iris (Oncorhynchus mykiss, Walbaum, 1792) en la región de Anatolia del sudeste de Turquía a través del cultivo bacteriológico y la reacción en cadena de la polimerasa. Además, también tenía como objetivo determinar la susceptibilidad antibiótica de la bacteria. Se realizaron visitas separadas a las instalaciones de cultivo de truchas en Diyarbakır, Adıyaman, Şanlıurfa y Batman en enero y febrero de 2021, y se recolectaron 30 peces con un peso vivo promedio de 200 a 250 g en cada instalación. Se tomaron un total de 1.200 muestras de 40 criaderos de truchas. Se recolectaron especímenes de hígado, bazo, riñón y tejido de trucha arcoíris. Se aisló Yersinia ruckeri del riñón, el hígado y el tejido de pescado en Agar McConkey para realizar exámenes bacteriológicos. Las cepas aisladas fueron identificadas por MALDİ–TOF y PCR. Se detectó el agente bacteriano en muestras de hígado, riñón y músculo tejido de peces en 23 de 40 granjas. En conclusión, estas bacterias fueron detectadas en granjas locales. Estas granjas deben implementar la vacunación, minimizar los factores de estrés que afectan a los peces y evitar el exceso de la sobre población. Los mejores métodos para protegerse contra la infección incluyen evitar la alta densidad los bajos niveles de oxígeno y la mano de obra poco calificada.

Citas

Ummey S, Khan S, Vijayakumar PPN, Ramya A. Enteric Red Mouth disease and its causative bacterium, Yersinia ruckeri, in Indian Major Carps from culture ponds in Andhra Pradesh, India. Aquacult. Fisheries. [Internet]. 2021; 6:289–299. doi: https://doi.org/kj3x

Wang D, Sun S, Li S, Lu T, Shi D. Transcriptome profiling of immune response to Yersinia ruckeri in spleen of rainbow trout (Oncorhynchus mykiss) l. BMC Genomics. [Internet]. 2021; 22:292. doi: https://doi.org/gjw9b2

Cao G, Yu Z, Zheng L, Lei X, Chen D. Isolation and identification of Yersinia ruckeri from hybrid sturgeon and pathological lesions of its infection. J. South China Agricult. Univ. [Internet]. 2018; 39(4): 13–19. doi: https://doi.org/kj3z

Mohammed–Saeed G. Causualty agent (yersiniosis) detection in reared rainbow trout (Oncorhynchus mykiss) in Kohgiluyeh– VA–boyerahmad province in Iran. Oceanogr. Fisheries Open Access J. [Internet]. 2017; 4(2): 555–633. doi: https://doi.org/kj32

Türkish Statistical Institute (TUİK). 2020; 60 p.

Türkish Statistical Institute (TUİK). 2021; 63 p.

Karabulut HA, Kurtoglu IZ. The Effect of Red Pepper to Add into Commercial Trout Feed on Growth Performance and Meat Quality of Rainbow Trout (Oncorhynchus mykiss). Fresenius Environm. Bull. 2017; 26: 7473–7477.

Wang D, Sun S, Li S, Lu T, Shi D. Transcriptome profiling of immune response to Yersinia ruckeri in spleen of rainbow trout (Oncorhynchus mykiss) l. BMC Genomics. [Internet]. 2021; 22:292. doi: https://doi.org/gjw9b2

Vega–Heredia S, Mendoza–Cano F, Sanchez–Paz A. The infectious hypodermal and haematopoietic necrosis virus: A brief review of what we do and do not know. Transb. Emerging. Dis. [Internet]. 2012; 59:95–105. doi: https://doi.org/ftkjr5

Adel M, Dawood MA, Shafiei S, Sakhaie F, Shekarabi SPH. Dietary Polygonum minus extract ameliorated the growth performance, humoral immune parameters, immune–related gene expression and resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). Aquacult. [Internet]. 2020; 519: 734–738. doi: https://doi.org/gmn4hw

Küçükköşker B. Üryani eriği (Prunus domestica) ekstraktinin gökkuşaği alabaliğinda (Oncorhynchus mykiss) kizilağiz hastaliğina (Yersinia ruckeri) karşi bağişiklik yaniti üzerine etkilerinin belirlenmesi. [Master's Thesis]. Turkey: Kastamonu Üniversitesi; 2000. 53 p.

Altinok I, Capkin E, Boran H. Comparison of molecular and biochemical heterogeneity of Yersinia ruckeri strains isolated from Turkey and the USA. Aquacult. [Internet]. 2016; 450: 80–88. doi: https://doi.org/kj7v

Karataş S, Candan A, Demircan D. Enteric redmouth disease in cultured rainbowtrout (Oncorhynchus mykiss) on the black sea coast of Turkey. Israeli J. Aquacult. Bamidgeh. 2004; 56 (3): 226–231.

Timur G, Timur M. Balık Hastalıkları . 5th. ed. İstanbul Üniversitesi. Su Ürünleri Fakültesi. 2003; 538 p.

Sibinga N.A, Marquis H. Tissue–specific differences in detection of Yersinia ruckeri carrier status in rainbow trout (Oncorhynchus mykiss). J. Fish Dis. [Internet]. 2021; 44:2013–2021 doi: https://doi.org/kj7x

Gibello A, Blanco MM, Morena MA, Cutuli MT, Domenench A, Dominguez L, Fernandez–Garayzabal JF. Development of a PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout. Appl. Environm. Microbiol. [Internet]. 1999; 65:346–350. doi: https://doi.org/kj7z

Kumar G, Menanteau–Ledouble S, Saleh M, El–Matbouli M. Yersinia ruckeri, the causative agent of enteric redmouth disease in fish. Vet. Res. 2015; 46(1):103.

Abdel–Latif HMR, Khalil RH, Saad TT, El–Bably RY. Identification and molecular characterization of Yersinia ruckeri isolated from mass mortalities of cultured Nile tilapia at Kafr El– Sheikh Governorate. Global J. Fisheries Aquacult. Res. 2014; 1: 17– 01

Mohammed–Saeed G. Causualty agent (yersiniosis) detection in reared rainbow trout (Oncorhynchus mykiss) in Kohgiluyeh– VA–boyerahmad province in Iran. Oceanogr. Fisheries Open Access J. 2017; 4: 555–633.

Pajdak–Czaus J, Platt–Samoraj A, Szweda W, Siwicki AK, Terechmajewska E. Yersinia ruckeri. A threat not only to rainbow trout. Aquacult. Res. 2019; 50: 3083–3096.

Zorriehzahra M, Adel M, Torabi –Delshad S. Enteric redmouth disease: Past, present and future: A review. Iranian J. Fisheries Sci. 2017; 17: 1135–1156.

Tinsley JW, Lyndon AR, Austin B. Antigenic and cross–protection studies of biotype 1 and biotype 2 isolates of Yersinia ruckeri in rainbow trout, Oncorhynchus mykiss (Walbaum). J. Appl. Microbiol. 2011; 111: 8–16.

Liu T, Wang KY, Wang J, Chen DF, Huang XL, Ouyang P, Geng YI, He Y, Zhou YI, Min J. Genome sequence of the fish pathogen Yersinia ruckeri SC09 provides insights into niche adaptation and pathogenic mechanism. Intern. J. Molec. Sci. 2016; 17(4): 557.