Caracterización filogenética y determinación de la susceptibilidad a los antibióticos de cepas patógenas de Escherichia coli aviar aisladas de órganos viscerales de pollos de engorde

  • Volkan Özavci Dokuz Eylül University, Faculty of Veterinary Medicine, Department of Microbiology. Kiraz, Izmir, Turkey https://orcid.org/0000-0003-3511-3008
  • Hafize Tuğba Yüksel-Dolgun Aydin Adnan Menderes University, Faculty of Veterinary Medicine, Department of Microbiology. Işıklı, Aydin, Turkey
  • Şükrü Kirkan Aydin Adnan Menderes University, Faculty of Veterinary Medicine, Department of Microbiology. Işıklı, Aydin, Turkey
Palabras clave: Pollos de engorde, biología molecular, Escherichia coli, genética, microbiología

Resumen

El estudio tuvo como objetivo identificar los grupos filogenéticos y la susceptibilidad a los antibióticos de los aislados de Escherichia coli de aves de corral (APEC). E. coli se caracterizó fenotípica y bioquímicamente a partir de hígado, 8/30 (26,66 %); corazón, 7/30 (23,33 %) y bazo, 4/30 (13,33 %) de pollos de engorde de 37-42 días de edad vacunados. Luego, los aislados de E. coli (19/90; 21,11 %) se filoagruparon mediante genotipado cuádruple en función de la presencia o ausencia de los genes arpA, chuA, yjaA y el fragmento de ADN TspE4.C2. La mayoría de las cepas APEC pertenecían al grupo filogenético C, seguido de los grupos A, E y F. El filogrupo C se observó en el hígado, el filogrupo A en las muestras de hígado y corazón, el filogrupo E en el corazón y el bazo y el filogrupo F en el hígado. La mayor resistencia antibiótica se observó en amoxicilina-ácido clavulánico y ampicilina (100 %) predominantemente en los grupos A y E según pruebas de susceptibilidad antibacteriana. También se encontró resistencia múltiple a antibióticos (MDR) para las cepas APEC en 68,42 % (13/19). De los 19 aislamientos probados, solo 13 (68 %) fueron susceptibles a niveles altos de gentamicina. Las cepas APEC pertenecientes a los filogrupos C, A y E son de importancia epidemiológica para los pollos de engorde. Sería beneficioso investigar nuevos filogrupos realizando análisis genotípicos más detallados en las cepas APEC.

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Publicado
2022-08-28
Cómo citar
Özavci, V., Yüksel-Dolgun, H. T., & Kirkan, Şükrü. (2022). Caracterización filogenética y determinación de la susceptibilidad a los antibióticos de cepas patógenas de Escherichia coli aviar aisladas de órganos viscerales de pollos de engorde. Revista Científica De La Facultad De Ciencias Veterinarias De La Universidad Del Zulia, 32, 1-8. https://doi.org/10.52973/rcfcv-e32159
Sección
Medicina Veterinaria