Genotyping of staphylococcus aureus isolated from dairy herds in México

• Hugo Castañeda Vázquez Universidad de Guadalajara-México
• Sybille J í¤ger Landesbetrieb Hessisches Landeslabor-Alemania
• Wilfried Wolter Landesbetrieb Hessisches Landeslabor-Alemania
• Michael Zsch í¶ck Landesbetrieb Hessisches Landeslabor-Alemania
• Martha Alicia Castañeda Vazquez Universidad de Guadalajara-México
• Amr El Sayed Cairo University-Egipto

Palabras clave: bovine mastitis, Staphylococcus aureus, molecular biology, enterotoxins, patogenic bacterius, genotipic relationship

Resumen

In the present work, Staphyloccous aureus field strains were isolated from 27 mastitic cows representing 12 dairy herds. This was selected of almost 3,000 field strains of mastitic cows. The strains were subjected to different Polymerase Chain Reaction (PCR) to detect the toxin encoding genes SEA, SEB, SEC, SED, SEE, SEG, SEJ and TST genes. The investigated strains were then subjected to fingerprinting by the means of Pulse Field Gel Electrophoresis (PFGE). The screening for the previously mentioned toxin encoding genes revealed the absence of all toxin encoding genes with the exception of SEI which could be detected in a single strain. Meanwhile, the data obtained through the PFGE analysis indicated the close relationship of S. aureus field strains responsible for the induction of mastitis in western Mexico.