Effect of db-cAMP on embryonic development of bovine oocytes fertilized with sex sorted semen

  • Hugo Hernández Fonseca Universidad del Zulia-Venezuela
  • Héctor Nava Trujillo Universidad del Zulia-Venezuela
  • Saksiri Sirisathien University of Georgia-Estados Unidos
  • Lawrence Johnson Unite State Department of Agriculture-Estados Unidos
  • David Guthrie Unite State Department of Agriculture-Estados Unidos
  • Glenn Welch Unite State Department of Agriculture-Estados Unidos
  • Adirmo Hernández Universidad del Zulia-Venezuela
  • Benjamin Brackett University of Georgia-Estados Unidos
Palabras clave: sorted sperm, db-cAMP, in vitro bovine embryos

Resumen

The objective of this study was to improve sexed bovine embryo production with sorted sperm in chemically defined conditions by supplementing the IVF medium with db-cAMP. Cumulus-oocyte complexes (COCs) were matured for 18 h in supplemented TCM-199 and fertilized with X- or Y-bearing sperm in the presence of heparin (10 μg/ml), db-cAMP (1 μM) or no treatment (control). Presumptive zygotes were cultured 54 h in g-SOF. From 72 to 144 h post- insemination (hpi) embryos were cultured in c-SOF+NEA and from 144 to 192 hpi embryos were placed in maturation medium without hormones. No significant differences were found among treatments for Y-sperm when compared to controls. A significant (P menor 0.01) improvement in the proportion of cleaved oocytes was found for X-sperm treated with db-Camp (70.83%) compared to the Y-sperm inseminated oocytes treated wit db-cAMP (46.37%). Treatment with db-cAMP enabled a better (P menor 0.05) blastocyst formation rate (19.29%) compared to control (8.47%) and heparin (10.44%). Treatment of db-cAMP significantly increased the rate of blastocysts in X-sperm inseminated oocytes (30.77%) compared to Y-sperm inseminated oocytes treated the same (9.68%) and compared to X- and Y-sperm treated with heparin (5.88% and 15.15%, respectively) and not treated (9.68% and 7.14%, respectively, P menor 0.05). These results suggest that db-cAMP may prove to be an effective treatment of sorted sperm for in vitro production of female bovine embryos under chemically defined conditions.

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Cómo citar
1.
Hernández Fonseca H, Nava Trujillo H, Sirisathien S, Johnson L, Guthrie D, Welch G, Hernández A, Brackett B. Effect of db-cAMP on embryonic development of bovine oocytes fertilized with sex sorted semen. Rev. Cient. FCV-LUZ [Internet]. 1 [citado 28 de marzo de 2024];17(2). Disponible en: https://produccioncientificaluz.org/index.php/cientifica/article/view/15267
Sección
Producción Animal