Comparison of Laboratory Techniques For the Diagnosis of Giardia Intestinalis

  • Marinella Calchi Parasitology. School of Bioanalysis. Faculty of Medicine. Zulia University
  • E. Acurero Parasitology. School of Bioanalysis. Faculty of Medicine. Zulia University
  • R. Villalobos Tropical Medicine School of Bioanalysis. Faculty of Medicine. Zulia University
  • M Colina Graduates in Bioanalysis. School of Bioanalysis. School of Medicine. Zulia University
  • L. Di Toro Graduates in Bioanalysis. School of Bioanalysis. School of Medicine. Zulia University
  • C. Villalobos Graduates in Bioanalysis. School of Bioanalysis. School of Medicine. Zulia University
Keywords: Giardiasis, diagnosis, microscopic examination with SSF-Lugol, Ritchie, Giardia-Strip

Abstract

Objective: To evaluate the sensitivity and specificity of three laboratory techniques for the diagnosis of Giardia intestinalis. Materials and Methods: 31 fecal samples from pre schoolers were submitted to microscopic examination with SSF-Lugol, the Ritchie concentration method and the “Giardia-Strip” immunological method. The Ritchie technique was considered the “gold standard.“ Results: G. intestinalis cysts were identified in 6 samples (19.35%) using the Ritchie technique. The fresh test identified 5 cases of protozoan cysts (16.1%), showing a sensitivity of 83 % and specificity of 100 %. The Giardia-Strip identified cysts in 4 samples (12.9%) with 66.66 % sensitivity and 100% specificity. The positive predictive value for the “Giardia Strip” method was 14% and the negative predictive value was 93%. The fresh test showed a positive predic tive value of 100% and a negative predictive value of 96 %. Conclusions: Ritchie concentrate and the fresh test showed higher sensitivity and specificity in the diagnosis of Giardia intestinalis, when samples contain only cysts. However, the Giardia-Strip method proved faster in obtaining results.

Published
2014-08-07
How to Cite
1.
Calchi M, Acurero E, Villalobos R, Colina M, Di Toro L, Villalobos C. Comparison of Laboratory Techniques For the Diagnosis of Giardia Intestinalis. Kasmera [Internet]. 2014Aug.7 [cited 2025Jul.22];42(1):32-0. Available from: https://produccioncientificaluz.org/index.php/kasmera/article/view/8653
Issue
Vol. 42 No. 1 (2014)
Section
Original Articles

Copyright (c) 2014 Marinella Calchi, E. Acurero, R. Villalobos, M Colina, L. Di Toro, C. Villalobos

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