Kinetic study of the N-deacetylation of chitin extracted from crustaceans’ exoskeletons employing ion chromatography

• Reinaldo Atencio Instituto Venezolano de Investigaciones Científicas (IVIC)
• Ana C. Valbuena Instituto Venezolano de Investigaciones Científicas (IVIC)
• Adrián Chavez Instituto Zuliano de Investigaciones Tecnológicas (INZIT)
• Guillermo Valbuena Instituto Zuliano de Investigaciones Tecnológicas (INZIT)
• Joan Vera-Villalobos Escuela Superior Politécnica del Litoral

DOI: https://doi.org/10.5281/zenodo.5593128

Keywords: Chitin, Chitosan, Kinetic, Deacetylation, Ionic Chromatography

Abstract

The chitin N-deacetylation kinetics from crustaceans’ exoskeletons was established in this work, following the acetate ion concentration as a reaction product. A single-stage deacetylation reactions was conducted at 100 °C in a NaOH at 50% m/v in m:v ratios of 5:10, 10:150, 15:150 and 20:150 mg:mL. The acetate ion concentration was determined using ionic chromatography each 10 min until 5 hours. Deacetylation degree (DD) influence, insoluble matter percentage, and ash content against variation of chitin quantities exposed in an alkali define volume was also studied. The increase of deacetylation degree was observed at higher ratios of m:v, in the same manner as insoluble matter and the ash content. These studies showed that single-stage deacetylation reaction has pseudo-first-order kinetics because the reaction rate correlated with the acetate group. Furthermore, it was established that from apart 150 min that all chitin:alkali ratios showed high deacetylation degrees. Multistep reactions lead to higher DD than those found for single-stage reactions.

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