Regeneration of Rubus in vitro using forchlorfenuron (CPPU)

B. Millán Mendoza


The ability of N-(2-chloro-4-pyridyl)-N-phenylurea(CPPU),a synthetic compound with cytokinin-like activity, to induce regeneration of whole plants in Rubus was assessed. Regeneration was obtained from internodal segments of three tissue culture recalcitrant red raspberry cultivars Glen Prosen (32%), Glen Moy (38%) and Glen Magna (72%) using media containing low concentrations of CPPU. The ability of CPPU to induce regeneration from the cultivars Taybeny (38%) and Loch Ness (46%) was examined and the results compared with previous work. The regeneration protocol here tested provides the first step for future genetic transformation involving these three Rubus varieties.

Palabras clave

CPPU; rubus; organogenesis; regeneration

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Science Citation Index (SCIExpanded), Scopus, Scimago Journal & Country Rank, Journal Citation Reports/Science Edition, Agris (FAO), CAB International (Reino Unido), Scielo Internacional, Latindex Catalogo, CIAT-Pastos Tropicales (Colombia), Revencyt (Venezuela), Tropical Abstracts (Holanda), Bionet (Venezuela), Weed abstracts, Serials Cited Index, SilverPlatter Information, Agroforestry Abstracts, Orton (México), Sistema de Información y Documentación Agrícola de Venezuela y Electronic Sites of Leading Botany, Plant Biology and Science Journals, Periódica (México), Dairy Science Abstracts, Field Crop Abstracts, Grasslands and Forage Abstracts, World Agricultural Economics and Rural Sociology Abstracts


Universidad del Zulia /Venezuela/Revista de la Facultad de Agronomía/  /p-ISSN: 0378-7818 / e-ISSN: 2477-9407


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